Figure 4. CSA concentration in mycelium and culture broth of endophyte K4 (A) (day 3–7) and in culture broth of five endophyte strains (day 5–25) (B).

The fungal Mycelium extracts treating by 80% ethanol and culture liquid were extracted by ethyl acetate three times. The evaporated ethyl acetate extracts were subsequently dissolved in chromatogram methanol for determination of CSA production by LC-MS/MS.