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. 2011 Nov 15;6(11):e27490. doi: 10.1371/journal.pone.0027490

Figure 2. Gal-3 Mediates K-Ras Localization and Gal-1 Mediates H-Ras Localization in Cell Membranes.

Figure 2

A. Gal-3 mediates K-Ras localization in the cell membrane. Selective mislocalization of GFP-K-Ras (12V) in Gal-3-/- MEFs but not in wt or Gal-1-/- MEFs. Cells were transfected with pEGFP-K-Ras (G12V) and were fixed after 48 h as described in Methods. Typical fluorescent confocal images of cultured cells of wt (upper panel) and of Gal-3-/- (lower panel) MEFs are shown. Bar, 5 µm. Data are presented as the mean fluorescence intensity of GFP-K-Ras (12V) in the plasma membrane (means ± SEM, N = 52 cells; wt, n = 12; Gal-1-/-, n = 14; and Gal-3-/-, n = 26, **p<0.001, ANOVA). B. Gal-1 mediates H-Ras localization in the cell membrane. Selective mislocalization of GFP-H-Ras (12V) in Gal-1-/- MEFs but not in wt MEFs. Experimental details are as in A except that pEGFP-H-Ras (G12V)-transfected Gal-1-/- MEFs were used. Means ± SEM are shown (wt, n = 14; Gal-1-/-, n = 12, **p<0.001, t-test). C. Typical background image of untransfected Gal-3-/- cells (1 of 3 MEFs examined). Similar images were obtained with the other cell lines used. MEFs, mouse embryonic fibroblasts; wt, wild type.