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. 2011 Nov 15;6(11):e27702. doi: 10.1371/journal.pone.0027702

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Matsuo, Roy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The recovery of BTV from RNA generated in vitro was compared using uncapped and capped ssRNA in the second transfection. BSR cells were transfected first with 6 capped ssRNAs (S1, S3, S4, S6, S8 & S9) and subsequently with all capped ssRNAs (column 1), all uncapped ssRNAs (column 2) or uncapped S9 together with remaining 9 capped ssRNAs (column 3). Each ssRNA was at 50 ng per transfection. The recovery of virus was shown as the total number of plaques per well (Mean ± SD).