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. 2011 Nov 15;6(11):e27734. doi: 10.1371/journal.pone.0027734

Table 3. Overview of Poecilia mexicana populations investigated, methods used, and number of specimens analyzed.

Population Habitat type Brain preparation/μ-CT of heads Orientation patterns (SEM, CLSM) 3D reconstruction of end organs Transversal semithin-sections of the saccule
Cueva del Azufre chamber XIII) cave creek free of hydrogen sulfide; complete darkness 3/1 4 1 2
Río Oxolotán sulfide-free river in the vicinity of the cave system; surface habitat 1/1 4 - 2
Tampico brackish coastal waters; surface habitat 2/- 1 1 2

Investigation of the orientation patterns of ciliary bundles of sensory hair cells was performed using either a scanning electron microscope (SEM) or a confocal laser scanning microscope (CLSM).