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. 2011 Nov 15;6(11):e27685. doi: 10.1371/journal.pone.0027685

Figure 5. In vivo effects of rmMFG-E8 on pSTAT3 and SOCS3 expression during endotoxemia.

Figure 5

WT mice were injected with rmMFG-E8 (0.4 µg/20 g BW) via i.p. for 2 h and then endotoxemia was generated by an overdose of LPS (5 mg/kg BW) administration via i.p route. After 2 h of LPS treatment mice were euthanized and total proteins were extracted from (A) peritoneal cells and (B) spleen to perform Western-blot using anti-pSTAT3/STAT3 Abs. On the other hand, SOCS3 expression was assessed by real-time PCR using each of the cDNAs extracted from above samples of (C) peritoneal cells and (D) spleen. β-actin served as an internal control. Data are expressed as means ± SE and compared by one-way ANOVA and SNK test from 3 independent experimental results. *P<0.05 vs. sham control; # P<0.05 vs. endotoxemia.