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. 2011 Nov 15;6(11):e27726. doi: 10.1371/journal.pone.0027726

Figure 1. Sag deletion sensitizes mES cells to all-trans Retinoid Acid (RA).

Figure 1

Two independent pairs of mES cells with genotypes of Sag+/+ and Sag−/− were seeded in 96-well plate in triplicate and treated with various concentrations of RA for 24 hrs. Cell viability was measured by ATP-lite assay. The IC50 curve was generated and IC50 value calculated by the Prism software (A). Cells were seeded in 6-well plate in duplicate and treated with DMSO vehicle control or RA (0.1 and 1 µM) for 6 days. The plates were stained (B) and the colonies with more than 50 cells were counted and plotted (C). Shown is x ± SEM from three independent experiments.