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. 2011 Nov 15;6(11):e27726. doi: 10.1371/journal.pone.0027726

Figure 6. Induction of SAG-SCF E3 ligase substrates by RA and/or MLN4924.

Figure 6

Cells were treated with indicated concentrations of MLN4924 for 24 hrs, followed by immunoblotting using cullin-1 antibody with β-actin as the loading control (A). Cells were treated with RA (1.5 µM), MLN4924 (0.1 µM for HL-60, 1.0 µM for KG-1) alone or in combination for 24 hrs, followed by immunoblotting using indicated antiobodies (B&C). mES cells were treated with RA at indicated concentrations for 24 hrs, followed by immunoblotting using NOXA antibody with β-actin as the loading control (D).