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Comparative sensitivity of a conventional heminested PCR and TaqMan PCR for detection of RV RNA. (A) Products of conventional PCR. The upper band shows the 397-bp product from the first-round reaction; the lower band shows the 376-bp product from the heminested reaction. (B) Results of TaqMan PCR. A Ct value of 40 indicates a negative reaction. Error bars show 95% CL of the mean of three replicate TaqMan PCRs.
