TABLE 2.

Archived paraffin wax-embedded tissues used to exclude cross-hybridisation of the Leishmania species probe

Infectious agent	Host	Tissue	Diagnostic method used for verification
Cryptosporidium species	Leopard gecko (Eublepharis macularius)	Small intestine	In situ hybridisation
Sarcocystis species	Sheep	Heart muscle	Histological examination
Eimeria species	Chicken	Intestine	Histological examination
Toxoplasma species	Manul (Felis manul)	Heart muscle	Immunohistochemistry
Giardia species	Rabbit	Intestine	Parasitological examination, in situ hybridisation
Entamoeba species	Boa constrictor (Boa constrictor)	Intestine	In situ hybridisation
Aspergillus	Humboldt penguin (Spheniscus humboldti)	Lung	Grocott’s methenamine silver stain
Candida	Gouldian finch (Erythrura gouldiae)	Gizzard	Grocott’s methenamine silver stain
CAV-2	Dog	Lung	In situ hybridisation, PCR
CPV-2	Dog	Intestine	Immunohistochemistry

CAV-2 Canine adenovirus type 2, CPV-2 Canine parvovirus type 2