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. 2004 Jan;42(1):347–350. doi: 10.1128/JCM.42.1.347-350.2004

FIG. 2.

FIG. 2.

Detection of SARS CoV from 18 subjects by real-time quantitative PCR. Data for RNA from 18 subjects are shown. The viral load is indicated as number of copies per ml of blood. Subject 1, 2,020 copies; subject 2, 9,100 copies; subject 3, 3,120 copies; subject 4, 9,600 copies. A sample would be considered positive if it generated a typical amplification curve within the 45 cycles. Negative signals from subjects 5 to 18 and the nontemplate control (water) are shown. The inset graph shows melting-curve analysis of the PCR products from the 18 subjects. The x axis denotes the temperature (oC), and the y axis denotes the fluorescence intensity (F2) over the background level.