TABLE 1.
Subject no. | Diagnosis | Days from onset | Days from illness to discharge or result | Result of quantitative real-time PCR (copies/ml of blood) | Result of gel-based nested PCR |
---|---|---|---|---|---|
1 | Probable SARS | 2 | 9 | 2,020 | + |
2 | Probable SARS | 4 | Died | 9,100 | + |
3 | Probable SARS | 4 | >30 | 3,120 | + |
4 | Probable SARS | 5 | 13 | 9,600 | + |
5 | Probable SARS | 12 | 18 | Negative | − |
6 | Probable SARS | 13 | 18 | Negative | − |
7 | Probable SARS | 14 | >30 | Negative | − |
8 | Probable SARS | 14 | 36 | Negative | − |
9 | Non-SARS | 0 | 0 | Negative | − |
10 | Non-SARS | 2 | 1 | Negative | − |
11 | Non-SARS | 3 | 12 | Negative | − |
12 | Non-SARS | 10 | 9 | Negative | − |
13 | Control | Negative | − | ||
14 | Control | Negative | − | ||
15 | Control | Negative | − | ||
16 | Control | Negative | − | ||
17 | Control | Negative | − | ||
18 | Control | Negative | − |
Eighteen subjects were tested by both nested PCR and quantitative real-time PCR (LightCycler). Four out of eight subjects with probable SARS cases tested positive. Specimens from non-SARS cases and controls from normal healthy individuals consistently tested negative.