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. 2004 Jan;42(1):347–350. doi: 10.1128/JCM.42.1.347-350.2004

TABLE 1.

Investigation of subjects for presence of SARS CoVa

Subject no. Diagnosis Days from onset Days from illness to discharge or result Result of quantitative real-time PCR (copies/ml of blood) Result of gel-based nested PCR
1 Probable SARS 2 9 2,020 +
2 Probable SARS 4 Died 9,100 +
3 Probable SARS 4 >30 3,120 +
4 Probable SARS 5 13 9,600 +
5 Probable SARS 12 18 Negative
6 Probable SARS 13 18 Negative
7 Probable SARS 14 >30 Negative
8 Probable SARS 14 36 Negative
9 Non-SARS 0 0 Negative
10 Non-SARS 2 1 Negative
11 Non-SARS 3 12 Negative
12 Non-SARS 10 9 Negative
13 Control Negative
14 Control Negative
15 Control Negative
16 Control Negative
17 Control Negative
18 Control Negative
a

Eighteen subjects were tested by both nested PCR and quantitative real-time PCR (LightCycler). Four out of eight subjects with probable SARS cases tested positive. Specimens from non-SARS cases and controls from normal healthy individuals consistently tested negative.