Fig. 2.

Verification of the ability of the assay to detect interspecies cross contamination. DNA from each species was mixed at a ratio of 5 ng:45 ng (order as labelled) or used as 50 ng input from a single species. DNA used was: WRC-256 (rat); 3T3 (mouse); H400 (human). PCRs were performed with the primer assays indicated. Amplified products generated at 32 cycles were detected by ethidium bromide staining of 1.5% agarose gels and UV transillumination. RNase free water was used as negative control. Arrows indicate the DNA molecular weight marker of 500 bp (Hyperladder IV; Bioline)