Table 1.
Information on the twelve reference genes used in this study
Gene | Full description | Gene function | Product size (bp) |
---|---|---|---|
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Glycolytic enzyme (Goossens et al. 2005) | 220 |
TUBB | Tubulin, beta polypeptide | Member of the tubulin family of structural proteins (Zhang et al. 2005) | 119 |
PPIA | Cyclophilin A | Protein folding and protein interaction (Thellin et al. 1999) | 114 |
ACTB | β-actin | Cytoskeletal structural protein (Goossens et al. 2005) | 188 |
YWHAZ | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide | Signal transduction by binding to phosphoserine-containing proteins (Goossens et al. 2005) | 248 |
RRN18S | 18S rRNA | Ribosome subunit (Goossens et al. 2005) | 120 |
B2M | Beta-2-microglobulin | Cytoskeletal protein involved in cell locomotion (Zhang et al. 2005) | 161 |
UBC | Ubiquitin C | Protein degradation (Perez et al. 2007) | 239 |
TBP | TATA box binding protein | General RNA polymerase II transcription factor (Goossens et al. 2005) | 174 |
RPLP | 60S acidic ribosomal protein PO | Structural protein of ribosomes (Lyng et al. 2008) | 150 |
GUSB | Beta-glucuronidase | Catalyses hydrolysis of β-d-glucuronic acid residues from the non-reducing end of mucopolysaccharides (Lyng et al. 2008) | 165 |
HPRT1 | Hypoxanthine phosphoribosyl-transferase I | Purine synthesis in salvage pathway (Goossens et al. 2005) | 94 |
The sizes of the PCR products of the reference genes were obtained from the manufacturer