Table 2.

The sequences of oligonucleotide primers used for detection of mycoplasmas

Primers of species-specific mycoplasmas	Primer sequence	Tm	GC	Amplicon size
Universal primer	S: GTG GGG AGG AAA YAG GAT TAG A	53–54.8	45–50	425 bp
	AS: GGC ATG ATG ATT TGA CGT CRT	50.5–52.4	45–48
M. Arginini	S: TGA TCA TTA GTC GGT GGA GAG TTC	55.7	46	326 bp
	AS: TAT CTC TAG AGT CCT CGA CAT GAC TC	58	46
M. Orale	S: TGA TCA TTA GTC GGT GGA AAA CTA	52.3	38	325 bp
	AS: TAT CTC TAG AGT CCT CGA CAT GAC TC	58	46
M. Hyorhinis	S: CGA TGA TCA TTA GTT GGT GGA ATA AAT	53.7	33	334 bp
	AS: AGG CAG TAT CTC TAG AGT CCT TAA CTT A	57	39
M. Fermentans	S: TGA TCA TTA GCT GAT GGG GAA CT	53.5	43	324 bp
	AS: TCT CTT AGA GTC CTC AAC TAA ATG	52.3	38
M. Genitalium	S: ATA GAT ACT AGC TGT CGG AGC GAT	55.7	46	335 bp
	AS: CCA ATT TAC ATT AGC AGT CTC GTT AA	53.2	35
A. Laidlawii	S: GAT GAG AAC TAA GTG TTG GCC ATA A	54.4	40	300 bp
	AS: CGC TAG AGT CCC CAA CTT AAT GA	55.3	48
M. Hominis	S: ATC ATT AGT CGG TGG AGA ATC A	55.1	41	301 bp
	AS: GCA GTA TCT CTA CTA GAG TCC TCA ACT TAAT	59.1	39
M. Pirum	S: TGG ATG TTA GAT GTC GGG GTA AA	53.5	43	324 bp
	AS: GTT GGC AGT ATC GCT AGA CAA A	56.7	41
M. Pneumoniae	S: GAT ACT AGC TGT CGG GGC GAT	56.3	57	329 bp
	AS: AAT TTG CAT TAG TAG CAG TCT CGC TAG	56.7	41
M. Salivarium	S: GAT CAT TAG TCG GCA GAG AAC TCG	57.4	50	324 bp
	AS: TAT CTC TAG AGT CCT CGA CAT GAC TC	58	46
U. Urealyticum	S: CAT CAT TAA ATG TCG GCT CGA A	51.1	41	323 bp
	AS: CGG TAG CAG TAT CGC TAG AAA AGC	57.4	50