FIG. 1.

Optimization of dipstick test conditions. The best result was obtained by using an antigen concentration of 750 μg/ml, a serum dilution of 1:50, and a conjugate dilution of 1:1,500. Under these conditions, samples from a negative control from an area where CanL is not endemic (A) and a negative control from an area of endemicity (B) are negative with the dipstick test, whereas samples from dogs with suspected (C), symptomatic (D), and confirmed (E) cases of CanL are positive with the dipstick test.