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. 2004 Jan;42(1):257–263. doi: 10.1128/JCM.42.1.257-263.2004

FIG. 1.

FIG. 1.

(A) Agarose gel electrophoresis and restriction analysis of the RT-LAMP product of the E gene of WN virus. Lanes: M, 100-bp DNA ladder (Sigma Genosys); 1, RT-LAMP with WN virus strain NY99; 2, RT-LAMP products digested with AluI (175 bp); 3, RT-LAMP without target RNA. (B and C) Comparative sensitivities of RT-LAMP and RT-PCR for detection of WN virus strain NY99 RNA. The amplification by RT LAMP (B) shows a ladder-like pattern, whereas the RT-PCR (C) shows a 201-bp amplification product. Lanes: M, 100-bp DNA ladder (Sigma Genosys); 1, 10,000 PFU; 2, 1,000 PFU; 3, 100 PFU; 4, 10 PFU; 5, 1 PFU; 6, 0.1 PFU; 7, 0.01 PFU; 8, 0.001 PFU; 9, 0.0001 PFU; 10, 0 PFU (negative control without target RNA).