TABLE 1.
Details of oligonucleotide primers used for RT-LAMP amplification of E gene of WN virus
| Primer name | Type | Length(s) | Genome positiona | Sequence (5′-3′) |
|---|---|---|---|---|
| F3 | Forward outer | 19-mer | 1028-1046 | TGGATTTGGTTCTCGAAGG |
| B3 | Reverse outer | 19-mer | 1228-1210 | GGTCAGCACGTTTGTCATT |
| FIP | Forward inner (F1C + TTTT + F2) | 46-mer; F1C, 22-mer; F2, 20-mer | F1C, 1121-1100; F2, 1050-1069 | TTGGCCGCCTCCATATTCATCATTTTCAGCTGCGTGACTATCATGT |
| BIP | Reverse inner (B1C + TTTT + B2) | 45-mer (B1C, 22-mer; B2, 19-mer) | B1C, 1144-1165; B2, 1208-1190 | TGCTATTTGGCTACCGTCAGCGTTTTTGAGCTTCTCCCATGGTCG |
| Loop F | Forward loop | 19-mer | 1093-1075 | CATCGATGGTAGGCTTGTC |
| Loop B | Reverse loop | 18-mer | 1169-1186 | TCTCCACCAAAGCTGCGT |
a
Genome position according to the WN virus strain NY99 (flamingo 382-99) complete genome sequence (GenBank accession number AF196835).