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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Nucl Med Biol. 2011 Aug 9;38(8):1183–1192. doi: 10.1016/j.nucmedbio.2011.05.005

Figure-3.

Figure-3

Reaction scheme showing the radiosynthesis of 18F-N-BOC-nifzetidine and 18F-nifzetidine. (A) Radioactivity and UV trace at 254 nm of HPLC analysis of 18F-N-BOC-nifezetidine intermediate using C18 reverse-phase semi-preparative column eluted with 60% acetonitrile- 40% 0.1M ammonium formate at a flow rate of 2.5 mL/min. Retention of 18F- N-BOC-nifzetidine was found to be 24.5 min. (B). Radioactivity and UV trace at 254 nm of HPLC purification of 18F-nifzetidine after two-step radiosynthesis using C18 reverse-phase semi-preparative column eluted with 60% acetonitrile- 40% 0.1M ammonium formate at a flow rate of 2.5 mL/min. Retention of 18F-nifzetidine was found to be 17.5 min.