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. Author manuscript; available in PMC: 2012 Dec 1.

Published in final edited form as: J Neurochem. 2011 Oct 20;119(5):1029–1040. doi: 10.1111/j.1471-4159.2011.07487.x

Figures 2A & 2B illustrate typical cell loss in db/+ and db/db mice brains by TTC staining following 48 hr H/I. The corresponding results are expressed as percentage area of infarction in Figure 2B. Data are mean ± SEM (n=23–24/ group). *p<0.05 vs db/+. db/+ ■ db/db

Figure 2C represents a typical zymogram obtained from extracts of contralateral (C) and ipsilateral (I) hemispheres of db/+ and db/db mice brain after 48 hr of hypoxic-ischemic recovery. A clear band was observed, at ~92kDa that corresponds to MMP-9 Fig. 2D illustrates significantly greater MMP-9 proteolytic activity in ipsilateral hemispheres of the db/db mice as compared to the db/+ mice. Results are expressed as mean ± SEM (n=3). Significance denoted as *p<0.05 vs db/+(C), # p<0.05 vs db/db (C). db/+(C) db/+(l) db/db (C) ■ db/db (l)

Figure 2E. Zymogram Analysis: Gradation of tissue damage.

Figure 2E provides a comparison of MMP-9 gelatinolytic activity in db/db and db/+ mice brains with different degrees of damaged tissue. Samples were selected based on the TTC measurement as described in Method section. Increased MMP-9 gelatinolytic activity is correlative with the percentage increase in infarction, but is significantly greater in the db/db. Results are expressed as mean � SEM (n=3–6/group). *p<0.05 vs db/+. db/+ ■ db/db

Figures 2A & 2B illustrate typical cell loss in db/+ and db/db mice brains by TTC staining following 48 hr H/I. The corresponding results are expressed as percentage area of infarction in Figure 2B. Data are mean ± SEM (n=23–24/ group). *p<0.05 vs db/+. db/+ ■ db/db

Figure 2C represents a typical zymogram obtained from extracts of contralateral (C) and ipsilateral (I) hemispheres of db/+ and db/db mice brain after 48 hr of hypoxic-ischemic recovery. A clear band was observed, at ~92kDa that corresponds to MMP-9 Fig. 2D illustrates significantly greater MMP-9 proteolytic activity in ipsilateral hemispheres of the db/db mice as compared to the db/+ mice. Results are expressed as mean ± SEM (n=3). Significance denoted as *p<0.05 vs db/+(C), # p<0.05 vs db/db (C). db/+(C) db/+(l) db/db (C) ■ db/db (l)

Figure 2E. Zymogram Analysis: Gradation of tissue damage.

Figure 2E provides a comparison of MMP-9 gelatinolytic activity in db/db and db/+ mice brains with different degrees of damaged tissue. Samples were selected based on the TTC measurement as described in Method section. Increased MMP-9 gelatinolytic activity is correlative with the percentage increase in infarction, but is significantly greater in the db/db. Results are expressed as mean � SEM (n=3–6/group). *p<0.05 vs db/+. db/+ ■ db/db