Repeated administration of low frequency (2 Hz) EA significantly decreased EtOH intake and preference. EA at low frequency (2 Hz) or sham EA were administered to two different groups of rats, on 6 consecutive days, 10 min before the start of EtOH- or water-drinking session. The effect was measured on day 1, 3, and 6. EtOH intake and preference was compared with the baseline drinking levels. There was no significant difference regarding the baseline drinking levels between the EA and the sham group. Significant reduction in EtOH intake (g/kg) and preference for EtOH during the 2 h EtOH access after the onset of drinking was found in EA (filled bars) but not in sham EA group (blank bars). The values are expressed as mean ± SEM. *p < 0.05, **p < 0.01 compared with baseline drinking levels, #p < 0.05, ##p < 0.01, ###p < 0.001compared with sham (two-way RM ANOVA followed by Newman-Keuls post hoc test), n = 6 animals in each group.