Figure 3. Dll1-K613R interacts with Mib1.
HEK293 cells were co-transfected with Mib1 and either DLL1-WT, DLL1-K613-618R, or DLL1-K613R plasmids as indicated. After 36 h, Mib1 was immunoprecipitated using anti-Flag antibody. Western blots were probed with anti-Flag or anti-Delta (sc-9102) antibodies to detect Mib1 or DLL1. Shown is a representative blot, n=3. IP, immunoprecipitation; WB, western blotting; CE, cell extract; WT, wild type.