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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Glia. 2011 Sep 21;60(1):69–82. doi: 10.1002/glia.21248

Figure 2. SIRT2 protein is severely reduced in the quakinviable brain and is rescued by transgenic expression of QKI6.

Figure 2

Representative Western blot analysis of SIRT2 expression in brainstem homogenates prepared from non-phenotypic heterozygous control (q/w), quakingviable mutant (q/q), and transgenic rescue mouse (q/qtg) at P10 (A), P20 (B) and P40 (C). SIRT2 is severely reduced at P10, P20 and P40 in the q/q brainstem as compared to that in q/w. Transgenic expression of QKI6 partially rescues the expression of SIRT2 at P10 (n=3) and completely restores it at P20 (n=1) and P40 (n=3). The bar graph shows the quantification of SIRT2 signals normalized to that of β-actin (mean±SD). The reduction of SIRT2 in q/q in comparison with that in q/w is significant in both P10 and P40 brain homogenates (n=3, p<0.01). The expression level of SIRT2 in q/qtg compared with that in q/q at P10 does not reach statistical significance, but is highly significant at P40 (p<0.01).