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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Sep 24;1807(12):1573–1582. doi: 10.1016/j.bbabio.2011.09.011

Figure 6. Inducible nitric oxide synthase (iNOS)-derived nitric oxide (˙NO) is required for chronic alcohol-induced liver hypoxia.

Figure 6

(A) Pimonidazole staining of formalin fixed liver sections from wild type and iNOS−/− mice with and without alcohol (EtOH) feeding was performed to assess liver hypoxia. (B) Quantification of the pimonidazole staining intensity from (A). Fluorescence microscopy was used to detect HIF-1α stabilization in liver sections from control and alcohol (EtOH)-fed wild type and iNOS−/− mice (C). The quantification of the immunofluorescence is shown in (D). Images are representative from each group and quantification results are mean ± SEM. n=6 per group. *p<0.05 compared to respective controls. #p<0.05 compared to EtOH-fed wild type mice.