Figure 7. Combination therapy of JO-1 and relaxin in the HCC1854 breast cancer model.

A) Schematic illustration of the experiment. Lethally irradiated mice received either mock transduced or LV-EF1a/Rlx transduced Lin^- bone marrow cells. Six weeks later, after engraftment of HSCs, mice were injected into the mammary fat pad with 4×10⁶ HCC1954 cells. Relaxin expression was activated by Doxycyclin 7 days later. Mice were then given weekly treatment of PBS, PBS/trastuzumab or JO-1/trastuzumab and tumor volumes were measured. B) Tumor volumes of individual mice. n=5. trastuzumab vs. JO-1 + trastuzumab P < 0.001; Rlx + JO-1 + trastuzumab vs. Rlx + trastuzumab P < 0.001; Rlx + PBS vs. PBS P < 0.001; Rlx + PBS vs Rlx +JO-1 + PBS P<0.001; Rlx +PBS vs Rlx + JO-1 + trastuzumab P<0.001