Figure 2.
N-acetylation of PABA in UV5/1A1 cells expressing CYP1A1 and NATb/NAT1*4 (solid bars) or NATa/NAT1*4 (open bars). (a) PABA N-acetylation activity following transient transfection with pcDNA5/FRT; (b) PABA NAT1 catalytic activity following stable transfection with pcDNA5/FRT of 3 different clones of each NATb/NAT1*4 and NATa/NAT1*4; (c) PABA N-acetylation activity following transient transfection with pEF1/V5-His; (d) PABA N-acetylation in situ following stable transfection of pcDNA5/FRT. Each bar represents mean ± S.E.M. for three transient transfections (a, c), 3 separate collections of 3 clones (b) or 3 separate collections of 1 clone (d). Asterisks (*) represent a significant difference (p<0.05) (a, b, d) or (p<0.0001) (c) following a student’s t-test.
