Table 1.

Primer Sequences

Primer Name	Use	Sequence
Lkm40P1	NATb 5-′UTR forward specific PCR	5′-GGCCGCGGCATTCAGTCTAGTTCCTGGTTGCC-3′
P1 Fwd Inr NheI	NATb 5′-UTR forward specific nested PCR	5′-TTTAAAGCTAGCATTCAGTCTAGTCTAGTTCCTGGTTGCCGGCT-3′
Lkm41P3	NATa 5′-UTR forward specifc PCR	5′-GGCCGCGGAACACATTCTGCTCAAATAAGCCT-3′
P3 Fwd Inr NheI	NATa 5′-UTR forward specific nested PCR	5′TTAATGCTAGCAACACATTCTGCTCAAATAAAGCCTAGG-3′
NAT (3′) ORF Rev	NATa/NATb 5′-UTR reverse PCR	5′-TTCCTCACTCAGAGTCTTGAACTCTATT-3′
NAT1 (3′) ORF For	NAT1 coding region forward PCR	5′-AGACATCTCCATCATCTGTGTTTACTAGT-3′
pcDNA5 FRTdistal Rev	NAT1 3′-UTR reverse PCR	5′-CGTGGGGATACCCCCTAGA
NAT1 KPN-Rev	NAT1 3′-UTR reverse nested PCR	5′-ATAGTAGGTACCTCTGAATTATAGATAAGCAAGATTCAGATTCT-3′
NATb Forward pEF1	NATb 5′-UTR forward specific pEF1a PCR	5′-AGTCGGATCCATTCAGTCTAGTTCCTGGTT -3′
NATa Forward pEF1	NATa 5′-UTR forward specific pEF1a PCR	5′-AGTCGGATCCAACACATTCTGCTCAAATAAAGCCTAGGCCAA-3′
NATa/b Reverse pEF1	NAT1 3′-UTR reverse pEF1a PCR	5′-TCGAGATATCAGCTCGGTACCTCTGAATTATAGA -3′
NAT1 total spliced Forward	NAT1 specific forward q-RT-PCR	5′-GAATTCAAGCCAGGAAGAAGCA-3′
NAT1 total spliced Reverse	NAT1 specific reverse q-RT-PCR	5′-TCCAAGTCCAATTTGTTCCTAGACT-3′
NAT1 TAQMAN probe for T.S.	TAQMAN probe for NAT1 total splice	6FAM-5′-CAATCTGTCTTCTGGATTAA-3′MGBNFQ