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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Neurochem. 2011 Oct 24;119(5):1137–1150. doi: 10.1111/j.1471-4159.2011.07499.x

Figure 2. Bax and VDAC1 oligomers identified in healthy neurons by 2-dimensional gel electrophoresis.

Figure 2

High K digitonin extracts were subjected to BN-SDS PAGE and blotted to PVDF membranes as detailed in the Methods section. The membranes were probed for Bax and VDAC1 with polyclonal N20 and N18 antibodies, respectively. (A) Representative images from two of five experiments are shown. Molecular mass markers for the first and second dimensions are indicated above and to the right, respectively, of the blots. Bax oligomers 1–5 (numbered) were routinely identified and closely resembled the pattern observed for the five indicated VDAC1 oligomers. The asterisks denote low molecular mass, presumably monomeric, Bax as the migration was similar to that seen when the extract was spiked with 1% SDS (B).