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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Neurochem. 2011 Nov 1;119(5):1074–1085. doi: 10.1111/j.1471-4159.2011.07513.x

Figure 4. Leu-enkephalin levels and delta opioid receptor activity in ECE-2 WT and KO spinal cord.

Figure 4

Levels of ir-Leu-enkephalin were examined by radioimmunoassay of spinal cord extracts from WT and KO mice without (A) and with (B) treatment with trypsin/carboxypeptidase B; no significant differences were observed. The levels of DPDPE-induced GTPγS binding in membranes from spinal cord extracts (C) were similar between genotypes. Data are represented as mean ±SEM from quadruplicate determinations from three individual animals (N=3 mice/genotype). DPDPE (D-Penicillamine(2,5)-enkephalin) is a delta opioid receptor agonist.