Table 1.
Neuropeptides identified from wild type mice spinal cord by MS/MS.
| Precursor | Peptide name |
Peptide sequence | Obs. Mass |
Theor. Mass |
Error (ppm) |
PEAKS Score |
LC-ESI-IT | LC-ESI-Q- TOF |
|---|---|---|---|---|---|---|---|---|
| Procholecystokinin | 23–32 | *QP.WPAEATDPV.E | 996.82 | 996.51 | 311 | 78 | X | |
| Proenkephalin | Met-enkephalin | KK.YGGFM.KR | 573.4 | 573.23 | 296 | 54 | X | |
| Proenkephalin | 197–208 | KR.SPQLEDEAKELQ.KR | 1385.99 | 1385.67 | 231 | 99 | X | |
| ProSAAS | little SAAS | R.SLSAASAPLVETSTPLRL.RR | 1812.66 | 1812.0 | 364 | 99 | X | |
| ProSAAS | PEN | RR.SVDQDLGPEVPPENVLGALLRV.KR | 2316.84 | 2316.23 | 263 | 99 | X | |
| Prosomatostatin | Somatostatin 28–14 | R.SANSNPAMAPRE.RK | 1243.79 | 1243.56 | 185 | 98 | X | |
| Protachykinin A | Neurokinin A | KR.HKTDSFVGL(AmideM).GKR | 1132.54 | 1132.57 | 26 | 45 | X | |
| Protachykinin B | Neurokinin B | KR.DMHDFFVGLMa.GKR | 1209.61 | 1209.52 | 74 | 97 | X | |
| Secretogranin 1 | 588–597 | KR.SFARAPQLDL.KR | 1117.01 | 1116.59 | 376 | 86 | X | |
| Secretogranin II | 569–581 | KR.IPVGSLKNEDTPN.R | 1382.59 | 1382.7 | 80 | 80 | X | X |
(.) indicates a cleavage site;
(*)
indicates the presence of signal peptide before the sequence;
“X” indicates the MS/MS platforms used to identify the peptide; Obs. mass, observed monoisotopic mass; Theor. mass, theoretical monoisotopic mass; putative PTM includes C-terminal amidation (“Amide”).