Immunoblot analysis of serum antibody reactivities to M. genitalium LAMP antigens among negative control and M. genitalium culture-positive women. Lane 1, negative control pooled sera; lanes 2 to 5, representative profiles from culture-positive individuals (corresponding ELISA values are given in parentheses). The band intensities of M. genitalium P146, P140, andP110 were scored as 1+ (lanes 2 and 3), 2+ (lane 4), and 3+ (lane 5) in comparison to the negative control.