TABLE 1.
M. genitalium-specific serological and PCR assessments at time of positive M. genitalium culture
| STD Patient no. | ELISA valuea | Immunoblot scoreb | PCR resultc |
|---|---|---|---|
| 6 | 0.05 | 1+ | − |
| 23 | 0.06 | 1+ | − |
| 26 | 0.08 | 1+ | + |
| 31 | 0.10 | 1+ | − |
| 13 | 0.12 | 1+ | − |
| 25 | 0.12 | 1+ | + |
| 14 | 0.13 | 1+ | − |
| 15 | 0.13 | 2+ | − |
| 22 | 0.13 | 1+ | − |
| 12 | 0.15 | 1+ | − |
| 4 | 0.18 | 1+ | + |
| 9 | 0.20 | 1+ | − |
| 29 | 0.20 | 1+ | − |
| 16 | 0.22 | 1+ | − |
| 10 | 0.25 | 1+ | − |
| 30 | 0.29 | 2+ | − |
| 2 | 0.30 | 2+ | + |
| 5 | 0.33 | 2+ | − |
| 17 | 0.33 | 2+ | + |
| 21 | 0.34 | 2+ | + |
| 19 | 0.40 | 2+ | + |
| 7 | 0.41 | 3+ | − |
| 1 | 0.42 | 1+ | − |
| 3 | 0.47 | 1+ | − |
| 11 | 0.56 | 1+ | + |
| 24 | 0.58 | 3+ | + |
| 27 | 0.62 | 3+ | + |
| 28 | 0.65 | 3+ | + |
| 8 | 0.69 | 3+ | − |
| 18 | 0.93 | 1+ | − |
| 20 | 1.56 | 2+ | + |
ELISA values are expressed as OD405.
Immunoblot scores are determined by subjective analysis of LAMP band intensities: 3+, strong; 2+, moderate; 1+, weak compared to negative controls (see Fig. 2).
PCR results are based upon presence (+) or absence (−) of predicted amplified product (374-bp fragment) by hybridization.