TABLE 1.
STD Patient no. | ELISA valuea | Immunoblot scoreb | PCR resultc |
---|---|---|---|
6 | 0.05 | 1+ | − |
23 | 0.06 | 1+ | − |
26 | 0.08 | 1+ | + |
31 | 0.10 | 1+ | − |
13 | 0.12 | 1+ | − |
25 | 0.12 | 1+ | + |
14 | 0.13 | 1+ | − |
15 | 0.13 | 2+ | − |
22 | 0.13 | 1+ | − |
12 | 0.15 | 1+ | − |
4 | 0.18 | 1+ | + |
9 | 0.20 | 1+ | − |
29 | 0.20 | 1+ | − |
16 | 0.22 | 1+ | − |
10 | 0.25 | 1+ | − |
30 | 0.29 | 2+ | − |
2 | 0.30 | 2+ | + |
5 | 0.33 | 2+ | − |
17 | 0.33 | 2+ | + |
21 | 0.34 | 2+ | + |
19 | 0.40 | 2+ | + |
7 | 0.41 | 3+ | − |
1 | 0.42 | 1+ | − |
3 | 0.47 | 1+ | − |
11 | 0.56 | 1+ | + |
24 | 0.58 | 3+ | + |
27 | 0.62 | 3+ | + |
28 | 0.65 | 3+ | + |
8 | 0.69 | 3+ | − |
18 | 0.93 | 1+ | − |
20 | 1.56 | 2+ | + |
ELISA values are expressed as OD405.
Immunoblot scores are determined by subjective analysis of LAMP band intensities: 3+, strong; 2+, moderate; 1+, weak compared to negative controls (see Fig. 2).
PCR results are based upon presence (+) or absence (−) of predicted amplified product (374-bp fragment) by hybridization.