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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Trauma. 2011 Nov;71(5):1288–1296. doi: 10.1097/TA.0b013e31822e2803

Figure 2.

Figure 2

Separation and identification of myeloid and lymphoid cells in the bone marrow through cell surface expression of lineage specific markers:

Total Bone marrow cells (1× 106 cells) were labeled with biotinylated antibodies to cell surface markers CD11b (specific myeloid cells) and CD19 (specific for lymphoid cells) and then incubated with APCy7 conjugated streptavidin and analyzed by flow cytometry. Those cells that express CD11b or CD19 were then separated through FACS sorting and stained with Wright - Giemsa stain to confirm their identity (magnification of 200X).