TABLE 1.
Oligonucleotide primers used to construct gene probes for DBCT of GBS isolates
| Primera | Target gene | Size of PCR amplicon (bp) | GenBank accession no.b | Annealing temp (°C) | Forward (top) and reverse (bottom) primer sequences |
|---|---|---|---|---|---|
| cpsIaH | cpsH | 759 | AB028896.2 | 54 | 5′-AACTCCTGATTTTGATAGAA-3′ |
| 5′-AGCAGGCCACTTTTGTAGAA-3′ | |||||
| cpsIaK | cpsK | 195 | AB028896.2 | 51 | 5′-ATTTATTTGTGACACTATCA-3′ |
| 5′-AATAGATAGACTTGTGTATC-3′ | |||||
| cpsIbH | cpsH | 307 | AB050723.1 | 47 | 5′-CGTTATTTAGAAGTCCAG-3′ |
| 5′-GGTATTATAAGATTTGAA-3′ | |||||
| cpsIIK | cpsK | 526 | NA | 55 | 5′-CCAACGGCAATAAAATACAT-3′ |
| 5′-GCATTGAGATTAGAGTAGTC-3′ | |||||
| cpsIIIH | cpsH | 389 | AF163833.1 | 45 | 5′-CTTTGGAAGAGTGAGTTTAG-3′ |
| 5′-TGACCAATTAGTGTAGCATA-3′ | |||||
| cpsIVM | cpsM | 408 | AF355776.1 | 53 | 5′-AGATTTGATTGGTTTTGTTG-3′ |
| 5′-CTATATGAGGAAGTTGTTGT-3′ | |||||
| cpsVO | cpsO | 185 | AF349539.1 | 53 | 5′-TTTTCCACATAATACATCTT-3′ |
| 5′-TAACCTTCTCCTTCACACTA-3′ | |||||
| cpsVIH | cpsH | 586 | AF337958.1 | 46 | 5′-AATGGATTCCTTCTATGA-3′ |
| 5′-ATTATCCTGTTTTGTTTG-3′ | |||||
| cpsVIIM | cpsM | 293 | NA | 54 | 5′-GTGCAATTAGAGGACAAAAA-3′ |
| 5′-CATCGAATCAGGAAAAATAG-3′ | |||||
| cpsVIIIJ | cpsJ | 570 | NA | 54 | 5′-AAACAAAGATAATGGTGGTC-3′ |
| 5′-GAAATAGCTGCCTGATAAAT-3′ |
a
Designations indicate the serotype from which the probe was amplified and the target gene.
b
NA, not available (C. E. Rubens, personal communication).