TABLE 2.

Oligonucleotide primers used for PCR amplification of 16S rRNA and urease A genes

Primer and sequence (5′-3′)a	Amplicon size (bp)	PCR conditions
16S rRNA
Outer primers
C70: AGAGTTTGATYMTGGC	1,500	94°C (45 s), 50°C (45 s), 72°C (45 s), with 5 s per cycle; 24 cycles
B37: TACGGYTACCTTGTTACGA
Inner primers
C97: GCTATGACGGGTATCC	400	94°C (1 min), 55°C (2 min), 72°C (3 min); 34 cycles
C98: GATTTTACCCCTACACCA
HP1: TGGCAATCAGCGTCAGGTAATG	500	94°C (1 min), 55°C (1 min), 72°C (3 min); 39 cycles
HP2: GCTAAGAGATCAGCCTATGTCC
Urease A
Outer primers
HPU1: GCCAATGGTAAATTAGTT	411	94°C (1 min), 45°C (1 min), 72°C (1 min); 35 cycles
HPU2: CTCCTTAATTGTTTTTAC
Inner primers
AGTTCCTGGTGAGTTGTTCT	361	96°C (30 s), 56°C (15 s), 74°C (30 s); 40 cycles
AGCGCCATGAAAACCACGCT

^aBase designations are standard International Union of Biochemistry designations for bases and ambiguity.