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Oligomeric states of AtPRMT10 mutants
| Monomer size (kDa) | GF size (−SAH) (kDa) | GF size/Monomer size | −SAH | +SAH (1 mM) | |||
|---|---|---|---|---|---|---|---|
| DLS size (kDa) | DLS size/Monomer size | DLS size (kDa) | DLS size/Monomer size | ||||
| wt | 43.1 | 80 | 1.8 | 93 | 2.2 | 92 | 2.1 |
| ΔN10 | 42.1 | N.D. | N.D. | 89 | 2.1 | 87 | 2.1 |
| ΔN20 | 41.4 | N.D. | N.D. | 83 | 2.0 | 83 | 2.0 |
| ΔN30 | 40.4 | 68 | 1.7 | 74 | 1.8 | 80 | 2.0 |
| Δ203-225 | 40.8 | 34 | 0.9 | 66 | 1.6 | 61 | 1.5 |
N.D., not determined; DLS, dynamic light scattering; GF, gel filtration
