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. 2011 Sep 19;50(46):9998–10012. doi: 10.1021/bi200998y

Figure 1.

Figure 1

Purity and circular dichroism spectra of insect cell-expressed and -purified Syt1. (A) Coomassie-stained 4 to 15% SDS–PAGE gel of affinity-purified, Hi5-expressed, full-length Syt1. (B) Circular dichroism spectra of full-length Syt1 in the presence and absence of Ca2+. (C) Circular dichroism spectra of the E. coli-expressed Syt1-C2AB fragment in the presence and absence of Ca2+.