. Author manuscript; available in PMC: 2012 Nov 11.

Published in final edited form as: Science. 2011 Nov 11;334(6057):806–809. doi: 10.1126/science.1207861

Table 1.

The kinetic parameters of Sirt5 on acetyl, malonyl, and succinyl peptides with different sequences.

Peptide		*k_cat* (s^-1)	K_m for peptide (μM)	*k_cat/K_m* (s^-1M^-1)
*H3 K9 (4-13 + WW^)**	deacetylation	ND	ND (>750)	7.8
	demalonylation	0.037 ± 0.003	6.1 ± 2.8	6.1 × 10³
	desuccinylation	0.025 ± 0.002	5.8 ± 2.7	4.3 × 10³
*GDH K503 (498-509 + WW^)**	deacetylation	ND	ND (>750)	<2^**
	demalonylation	0.014 ± 0.001	8.7 ± 1.3	1.6 × 10³
	desuccinylation	0.028 ± 0.002	14 ± 4	2.0 × 10³
*ACS2 K628 (623-632 + WW^)**	deacetylation	ND	ND (>750)	18
	demalonylation	0.079 ± 0.008	150 ± 40	5.2 × 10²
	desuccinylation	0.268 ± 0.051	450 ± 150	6.0 × 10²

Two tryptophan residues were added at the C-terminal of the peptide to facilitate the detection by UV-Vis absorption during the HPLC assay.

^**

No activity. The value was estimated based on the detection limit.

ND: cannot be determined either because no activity was observed or because V versus [S] was linear (even the highest [S] of 750 μM used was much smaller than the K_m).