Fig. 3.

Confirmation of microarray results by quantitative RT-PCR using select genes involved in protein degradation. mRNA expression levels of cathepsin K (Ctss, A), matrix metalloproteinase 2 (Mmp2, B), calpain 2 (Capn2, C), lysosomal-associated protein transmembrane 5 (Laptm5, D), F-box protein 32 (Fbxo32, E), and tripartite motif-containing 63 (Trim63, F) in the gastrocnemius muscle of mice whose muscles where immobilized by casting or tenotomy. Values represent the mean relative expression ± SD; n = 8 for microarray and n = 3 for qRT-PCR.