Hippocampal CpG methylation of DUSP5 in 5′-flanking SP1Pr region and exon 2 (Ex2). A: schematic diagram of the DUSP5 gene 5′-region of Ex2. Numbers indicate the position relative to the transcription start site. The position of the 6 CpG sites on the 5′-flanking promoter and 5 CpG sites on Ex2 detected by bisulfite sequencing is indicated by a short vertical bar. B: percentage of methylation ± SE of 6 CpGs on the 5′-flanking SP1Pr region and 5 CpGs on Ex2 of the DUSP5 gene in rat brain from male at DOL0 (B, C), female at DOL0 (D, E), male at DOL21 (F, G) and female DOL21 (H, I). IUGR values are presented as black diamonds, and Con values are presented as squares. To the right of each graph is the methylation pattern of 14 representative control and IUGR on 5′-flanking SP1Pr region and Ex2 clones. Methylated CpGs are filled and nonmethylated CpGs are open. **P < 0.01, *P < 0,05.