Table 1.
Validation of gene expression changes by quantitative RT-PCR
| Gene | Analysis list* | Test |
|---|---|---|
| t test | (E,L) | |
| FANCD2 | Diagnosis vs relapse | 0.011 |
| FOXM1 | Diagnosis vs relapse | 0.002 |
| HRK | Diagnosis vs relapse | 0.590 |
| CENPM | Diagnosis vs relapse | 0.017 |
| SMEK2 | Diagnosis vs relapse | 0.140 |
| IFIT5 | Diagnosis vs relapse | 0.110 |
| LAPTM4B | Diagnosis vs relapse | 0.280 |
| OBSL1 | Diagnosis vs relapse | 0.002 |
| CCNB2 | Early relapse | 0.055, 0.089 |
| KCNH2 | Early relapse | 0.013, 0.065 |
| TYMS | Late relapse | 0.140, 0.028 |
| ATIC | Late relapse | 0.028, 0.009 |
| CAD | Late relapse | 0.051, 0.035 |
| GART | Late relapse | 0.062, 0.034 |
| PAICS | Late relapse | 0.037, 0.013 |
| IMPA2 | Late relapse | 0.030, 0.001 |
| RAD51AP1 | Late relapse | 0.100, 0.002 |
| BIRC5 | Early and late relapse | 0.005 |
| BUB1B | Early and late relapse | 0.018 |
| Wilcoxon test† | ||
| WT1 | M/E | 0.05 |
| GATA5 | M/E | 0.05 |
| CSMD1 | M/E | 0.05 |
| COL6A | M/E | 0.05 |
| CDKN2A | M/E | 0.05 |
| TOP1MT | M/E | > 0.05 |
| NOTCH4 | M/E | > 0.05 |
E,L indicates diagnosis vs early relapse, diagnosis vs late relapse; and M/E, correlation of methylation and gene expression.
*
All comparisons are from diagnosis to relapse.
†
Wilcoxon paired signed-rank test.