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. Author manuscript; available in PMC: 2011 Nov 16.
Published in final edited form as: Mol Cell. 2009 Sep 24;35(6):755–768. doi: 10.1016/j.molcel.2009.09.015

Figure 5. MED1-dependent recruitment of the PGC-1α C-terminus and PGC-1α LXXLL mutants to the TRα-RXRα-UCP-1 enhancer complex.

Figure 5

In panels B, C and D, standard immobilized template recruitment assays with purified proteins and T3 added as indicated were carried out as described in Experimental Procedures and bound proteins were detected by immunoblot.

(A). Wild type and mutant PGC-1α proteins. The upper panel shows a schematic representation of the full length, truncated and triple LXXLL-mutated proteins. The lower panel shows an SDS-PAGE analysis (with Coomassie blue staining) of the purified His-tagged proteins.

(B). Selective T3-dependent binding of an N-terminal PGC-1α fragment to the TRα-RXRα-UCP-1 enhancer complex.

(C). MED1-dependent binding of C-terminal PGC-1α fragments to the TRα-RXRα-UCP-1 enhancer complex.

(D). MED1- and T3-dependent, LXXLL motif-independent binding of full length PGC-1α to the TRα-RXRα-UCP-1 enhancer complex.