Table 1. Genes showing ≥5-fold induction by both 1 mM GSNO and 1 mM NaNO2.
| GSNO
|
NaNO2
|
|||||
|---|---|---|---|---|---|---|
| Gene | b no. | 0.1 mM | 1 mM | 0.1 mM | 1 mM | Function |
| norV | b2710 | 173 | 85 | 75 | 78 | Flavorubredoxin |
| norW | b2711 | 338 | 98 | 169 | 52 | Flavorubredoxin reductase |
| hmpA | b2552 | 70 | 37 | 53 | 31 | Flavohemoglobin |
| ytfE | b4209 | 38 | 29 | 21 | 50 | Homology to NO-dependent regulators |
| soxS | b4062 | 2 | 27 | 2 | 36 | Regulator of soxRS regulon |
| yftA | b2597 | 3 | 19 | 2 | 22 | Homology to σ54 modulators |
| fes | b0585 | 11 | 15 | 14 | 17 | Enterochelin esterase |
| yhjX | b3547 | 2 | 14 | 3 | 9 | Homology to oxalate/formate antiporters |
| nrdH | b2673 | 17 | 14 | 18 | 10 | Glutaredoxin-like protein |
| nrdI | b2674 | 18 | 8 | 19 | 5 | Probably involved in ribonucleotide reductase function |
| ybaE | b0445 | 1 | 14 | 1 | 9 | Putative ABC-type transporter |
| ychH | b1205 | 1 | 11 | 1 | 9 | Putative membrane protein |
| hofF | b3327 | 6 | 11 | 7 | 11 | Putative general secretory pathway protein |
| ygbA | b2732 | 5 | 10 | 4 | 8 | Conserved hypothetical protein |
| tdcB | b3117 | 2 | 10 | 2 | 7 | Threonine dehydratase |
| uspA | b1895 | 1 | 9 | 1 | 6 | Universal stress protein A |
| hycI | b2717 | 15 | 8 | 14 | 6 | Hydrogenase 3 maturation protease |
| ybhG | b0795 | 2 | 8 | 2 | 7 | Homology to HlyD-family secretion proteins |
| sufA | b1684 | 6 | 7 | 7 | 7 | Homology to iron-binding protein IscA |
| yadG | b0127 | 7 | 7 | 6 | 10 | Putative ABC-type transporter |
| fhuF | b4367 | 9 | 7 | 9 | 10 | Ferric iron reductase protein |
| yqeB | b2875 | 1 | 6 | 1 | 5 | Homology to xanthine and CO dehydrogenases maturation factors |
| yhaK | b3106 | 1 | 6 | 1 | 25 | Homology to pirin-related proteins |
| yjjM | b4357 | 1 | 6 | 1 | 9 | Homology to transcriptional regulators |
| yfiD | b2579 | 1 | 6 | 1 | 6 | Homology to formate acetyltransferases |
| ybaR | b0484 | 2 | 6 | 4 | 7 | Homology to copper-transporting P-type ATPase |
| asnA | b3744 | 4 | 6 | 4 | 5 | Asparagine synthetase A |
| deoC | b4381 | 1 | 6 | 1 | 5 | Deoxyribose-phosphate aldolase |
| ilvC | b3774 | 2 | 6 | 2 | 8 | Ketol-acid reductoisomerase |
| ybiJ | b0802 | 2 | 6 | 2 | 44 | Conserved hypothetical protein |
| ascF | b2715 | 2 | 6 | 1 | 6 | Phosphotransferase system (PTS) system enzyme IIABC |
| ycgT | b1200 | 2 | 5 | 1 | 5 | Homology to dihydroxyacetone kinase |
| adhE | b1241 | 2 | 5 | 2 | 6 | Aldehyde-alcohol dehydrogenase |
| ycfR | b1112 | 2 | 5 | 2 | 17 | Conserved hypothetical protein |
Columns show average of induction ratios for two independent RNA samples isolated from MG1655 cells left untreated or exposed to 0.1 or 1 mM concentrations of GSNO or NaNO2 for 5 min. For all induction ratios ≥2, the P values are <0.05. The norVW and nrdHI genes, which are contained within operons, are grouped together. For most genes within operons, all genes show induction, though the induction ratios decrease for the downstream genes. Descriptions of function are taken from http://genolist.pasteur.fr/Colibri and/or www.ncbi.nlm.nih.gov/BLAST.