Fig. 4.
Effect of DEHP treatment on estrogen biosynthesis. Serum E2 levels (A) were higher due to increased Leydig cell E2 production (B and C) and increased aromatase gene expression (D) after DEHP treatment of rats from 21 to 48 days of age (n = 10). E2 production was normalized to nanograms per 106 cells and measured by RIA in aliquots of the spent media after incubation of Leydig cells for 3 h. S16, rat ribosomal protein S16; *, P < 0.01 compared to control. Data are presented as mean ± SEM.