Figure 5.

(a), (b) Liver of the control group. (a) H and E staining, ×200 magnification. In Zone3-Zone2, moderate fatty changes were observed. Ballooning degeneration was sporadically observed. (b) TNF-α immunostaining, ×400 magnification. No TNF-α expression was seen in hepatocytes or Kupffer cells. (P: portal tracts, CV: central vein) (c), (d) Liver of the HBOT group. (c) H and E staining, ×200 magnification. Fatty changes were less severe, while marked ballooning degeneration was observed. Mallory bodies were seen sporadically (inset). (d) TNF-α immunostaining, ×400 magnification. TNF-α expression was observed in the hepatocytes of the perivenular and periportal areas; however, it was not seen in Kupffer cells. (P: portal tracts, CV: central vein).