Table 2. Interval and regularity of spacing of IcsA507–620-GFP foci.
| Strain background | Relevant genotype | Region of filaments analyzed | Distance between IcsA507-620-GFP foci, mean ± SD in μm (N) |
|---|---|---|---|
| MC4100 | WT* | All | 2.1 ± 0.5 (109) |
| PB103 | WT* | All | 2.7 ± 1.2 (40) |
| EC307 | MC4100 ftsZ84 (Ts) | All | 2.6 ± 0.5 (349) |
| PB114 | PB103 ΔminCDE | All | 2.6 ± 0.7 (209) |
| AJ35 | PB103 ftsZ84 (Ts) ΔminCDE | All | 3.3 ± 1.2 (246) |
| AJ41 | PB103 mukB | All | 2.6 ± 1.1 (243) |
| Nucleate | 2.4 ± 1.1 (103) | ||
| Anucleate | 2.3 ± 1.2 (39) | ||
| WC1016 | PB103 ftsA12 (Ts) dnaA5 (Ts) | All | 2.9 ± 1.2 (307) |
| Nucleate | 2.5 ± 0.8 (44) | ||
| Anucleate | 2.2 ± 1.1 (80) | ||
| AJ46 | PB103 ΔminCDE dnaA5 (Ts) | All | 3.9 ± 1.7 (216) |
| Nucleate | 3.6 ± 1.3 (69) | ||
| Anucleate | 3.2 ± 1.2 (73) |
*
WT strains were filamented by treatment with aztreonam (see Materials and Methods).