Fig. 5.

sdeC is required for efficient intracellular growth. D. discoideum cells were infected with a multiplicity of infection of 0.05, and growth of bacteria was monitored as described (13). Total bacterial cells were washed from individual microtiter wells at designated times, and the appropriate dilutions were plated on charcoal yeast extract plates to obtain the colony-forming units. Fold of growth was obtained by dividing colony-forming units at a given time point by the input bacterial cell numbers. Strains tested are as follows: black bars, Lp02(intact dot/icm); gray bars, Lp02(ΔsdeC); striped bars, Lp02(ΔsdeC) harboring pZL192 that carries the ORF of sdeC. Data shown are from two independent experiments performed in triplicate.