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. Author manuscript; available in PMC: 2012 Nov 11.
Published in final edited form as: J Mol Biol. 2011 Sep 21;413(5):1016–1027. doi: 10.1016/j.jmb.2011.09.029

Table 1.

Dissociation constants for the binding of promoter fragments to RNAP beacon, RNAP beacon complex with gp2 and Δβ′(1149–1190) RNAP beacon.

DNA probe Kd, RNAP, μM Kd, (RNAP- gp2), μM Kd, (RNAP- β′Δjaw), μM
[−12/+20][+4/+20] <10−4 0.12 3.4–10−3
NC(−4/+3) <10−4 >0.6 1.0–10−3
−12/+2 0.16 4.4 0.46
−12/−3 1.8 17
−12/−6 80 87

The structures of DNA probes are shown in Figs. 3A, 4A and 5. The Kd values presented are averages from 2–3 individual experiments; the error is ±15%.