HeLa cells were transfected with MMP1, PRUNE, or UGT2B15 luciferase reporter vectors with or without an expression vector for ERα, as indicated. The cells were then treated with Ral (100 nM) or E2 (100 nM), as indicated, before determination of luciferase activity. Each bar represents the mean ± SEM for n = 3 separate experiments. Bars marked with an asterisk are significantly different from the corresponding controls (Student’s t-test, p < 0.05).