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. Author manuscript; available in PMC: 2013 Jan 2.
Published in final edited form as: Mol Cell Endocrinol. 2011 Sep 22;348(1):331–338. doi: 10.1016/j.mce.2011.09.032

Fig. 4. JNK kinase activity is required for Ral-dependent activation of the MMP1 and PRUNE promoters in reporter gene assays.

Fig. 4

HeLa cells were transfected with MMP1 or PRUNE luciferase reporter vectors and an expression vector for ERα. The cells were then treated with the JNK inhibitor SP (20 μM) for 1 hour prior to treatment with Ral (100 nM) or E2 (100 nM), as indicated, before determination of luciferase activity. Each bar represents the mean ± SEM for n ≥ 3 separate experiments. Bars marked with an asterisk are significantly different from the corresponding controls (Student’s t-test, p < 0.05).