Table 2.
Oligonucleotides used in this study
| Name | Sequence (5'→3') | Purpose |
|---|---|---|
| Expression vectors | ||
| s5526 | AAAAAAGCTAGCGGCTCCTGAAACCAGGAG | Amplification of PmtlA |
| s5527 | AAAAACTTAAGATATAAACCCTCCCTGTTTTG | Amplification of PmtlA |
| s5799 | AAGCTAGCTACGATATTCCATAAAAAGC | Amplification of PmtlR |
| s6209 | AAAAAGCTAGCTTTTTATTTTTAAAAAATTGTCACAGTCA | Shortening upstream of PmtlA |
| s6210 | AAAAAAGCTAGCTAAAAAATTGTCACAGTCATGTGC | Shortening upstream of PmtlA |
| s6213 | AAAAAACTTAAGTAAGATACAAAAATATGTTCAGAGA | Shortening downstream of PmtlA |
| s6392 | AAAAAACTTAAGAGCCAATCTTGATGTGCGG | Amplification of PmtlR |
| s6688 | AAAAAAGCTAGCTTTTTATTTTTAAAAAATTGTCACAGTCATGTGCCAAAGTCCTGAAATCTTTCAA TTGTATAGGGACTG | Mutation between -35 and MtlR binding site |
| s6726 | AAAAAAGCTAGCTATTTTTAAAAAATTGTCACAGT | Shortening upstream of PmtlA |
| s6727 | AAAAAACTTAAGAGAGAATGATGCTTCCCTTTG | Shortening downstream of PmtlA |
| s6728 | ATACAATTCTTTCTAAAGAGGACTTTGGCACATG | Mutation between -35 and MtlR binding site |
| s6729 | CCTCTTTAGAAAGAATTGTATAGGGACTGTAAGCGT | Mutation between -35 and MtlR binding site |
| s6792 | AAAAAAGCTAGCTTTATTTTTAAAAAATTGTCACA | Shortening upstream of PmtlA |
| s6793 | AAAAAAGCTAGCTTTTTAAAAAATTGTCACAGTC | Shortening upstream of PmtlA |
| s6800 | CGCTTACAGTCCCTATACAAAACTTAGTAAAGAGGACTTTGGCAC | Mutation between -35 and MtlR binding site |
| s6801 | CCAAAGTCCTCTTTACTAAGTTTTGTATAGGGACTGTAAGCG | Mutation between -35 and MtlR binding site |
| s6829 | AAAAAAGCTAGCTTTAAAAAATTGTCACAGTCAT | Shortening upstream of PmtlA |
| s6830 | AAAAAAGCTAGCAAAAATTGTCACAGTCATGTG | Shortening upstream of PmtlA |
| s7065 | AAAAAAGCTAGCTTTTTATTTTTAAAAAATTGTCACAGTCATGTGCCAAACAGGACTTTACTTTCAA TTGTATAGGG | Mutation between -35 and MtlR binding site |
| s7091 | AAAAAAGCTAGCTTTTTATTTTTAAAAAATTGTCACAGTCATGTGGGTTTGTCCTCTTTACTTTCAA TTGTATA | Mutation between -35 and MtlR binding site |
| s7098 | AAAAAAGCTAGCAGCTATTGTAACATAATCGGT | Fusion of PgroE-(cre)-UTRPmtlR |
| s7189 | CCTTAAAACGCTTACAGCAATTCTTATAATAAAGAATCTCC | Fusion of PgroE-(crePmtlA)-UTRPmtlR |
| s7190 | TGCTGTAAGCGTTTTAAGGAAACCTCTCTATATCCTCTA | Fusion of PgroE-(crePmtlA )-UTRPmtlR |
| s7191 | CCATAAAACGCTTTCAACAATTCTTATAATAAAGAATCTCC | Fusion of PgroE-(crePmtlR)-UTRPmtlR |
| s7192 | TGTTGAAAGCGTTTTATGGAAACCTCTCTATATCCTCTA | Fusion of PgroE-(crePmtlR )-UTRPmtlR |
| s7193 | CCTGGTAACGCTTTCAACAATTCTTATAATAAAGAATCTCC | Fusion of PgroE-(creacsA)-UTRPmtlR |
| s7194 | TGTTGAAAGCGTTACCAGGAAACCTCTCTATATCCTCTA | Fusion of PgroE-(creacsA )-UTRPmtlR |
| s7195 | CCTGTTCACGCTTTCAGCAATTCTTATAATAAAGAATCTCC | Fusion of PgroE-(cremtlA)-UTRPmtlR |
| s7196 | TGCTGAAAGCGTGAACAGGAAACCTCTCTATATCCTCTA | Fusion of PgroE-(cremtlA )-UTRPmtlR |
| s7237 | GTAGAGGATATAGAGAGGTTTCCCAATTCTTATAATAAAGAATCTCC | Fusion of PgroE-UTRPmtlR |
| s7238 | GGAGATTCTTTATTATAAGAATTGGGAAACCTCTCTATATCCTCTAC | Fusion of PgroE-UTRPmtlR |
| Integration vector | ||
| s5069 | AAAAAAGAATTCGATATCAGATCTACGCGTTAACCCGGGC | Amplification of ermC |
| s5070 | AAAAAACAATTGAATCGATTCACAAAAAATAGG | Amplification of ermC |
| s5621 | AAAAAAGGCGCCTGGATTACCGTCTCATCG | Amplification of glcT |
| s5622 | AAAAAAGGATCCAACCGCTTCCGCCTCATGAA | Amplification of glcT |
| s5623 | GTGTTAGTACGCCGTGCTT | Amplification of ptsHI |
| s5624 | GTCGCAATCATAGGGAACAT | Amplification of ptsHI |
| s5809 | AAAAAAGATATCAACGCCCTTGCCCTTTC | Amplification of ycsN |
| s5810 | AAAAAAAGATCTGCATCAGCTGGTAAACTGAT | Amplification of ycsN |
| s5812 | AAAAAAAGGCCTAACACAAATGTTGTTTCTGC | Amplification of ydaB |
| s5860 | AAAAAAACTAGTACCTGCATGGCACACGT | Amplification of ydaB |
| s5866 | AAAAAAGGATCCATAAGAATTGACCTCCTCT | Amplification of glcT-PptsGHI |
| s5867 | AAAAAAGGATCCTAAGGGTGTTAGTACGCCGT | Amplification of ptsHI |
| s5918 | AAAGATCTAACCAGGAGCCTTTTTATTTT | Amplification of PmtlA |
| s5919 | CGAAATGTAAGGCGATCATATATAAACCCTCCCTGTT | Amplification of PmtlA |
| s5920 | AACAGGGAGGGTTTATATATGATCGCCTTACATTTCG | Amplification of mtlD |
| s5921 | AAGATATCGACCGTAAACAGCTTCCGTT | Amplification of mtlD |
| s5994 | AAACTAGTAAGAAACTTAATCAATAACCGAC | Amplification of ycsA |
| s5995 | AAAGGCCTTCTCGATTCCGCTATAATCAG | Amplification of ycsA |
| s6067 | CCTGAAAGAAACACCATGCCCGAAC | Amplification of ycnL |
| s6068 | AAGATATCGAAAGAAACACCATGCCCGAAC | Amplification of ycnL |
| s6079 | AAAAAAACTAGTCTTTGGCACATGACTGTGACA | Amplification of ycnL |
| s6080 | AAAGATCTCTTTGGCACATGACTGTGACA | Amplification of ycnL |
| s6302 | AAAAAAGAATTCGGTATCTATCTTTTATGCCAA | Amplification of ydjC |
| s6303 | AAAAAAGCTAGCTACGTAGTTCTGTCAGCAATC | Amplification of ydjC |
| s6304 | AAAAAACTTAAGATCATTGAAGATGTTTCTTGA | Amplification of pspA |
| s6305 | AAAAAACATATGCAGCAATTTGATTCGCCGC | Amplification of pspA |
| s6344 | AAAAAAGATATCGATCGCCTTACATTTCGGTGC | Amplification of mtlD |
| s6345 | AAAAAACATATGTTAAAATGATGGCGTGCAACG | Amplification of mtlD |
| s6865 | GCTGACGGCCGGCTCCAGATCT GCA ATC AAG CCT TCA TAT AA | Amplification of mtlR-H342D |
| s6866 | TTATATGAAGGCTTGATTGCAGATC TGG AGC CGG CCG TCA GC | Amplification of mtlR-H342D |
| s6867 | AAAAAA ACTAGT TTA CAG TAT GTT TTT TTC TTT CAT | Amplification of mtlR-H342D |
| s6949 | AAAAAA CCCGGG TAC GAT ATT CCA TAA AAA GC | Amplification of mtlR-H342D |
| Primer extension | ||
| s5959 | Cy5-GCTGCAAGGCGATTAAGTTGG | Hybridized to lacZ |
| s5960 | Cy5-CCAGTCACGACGTTGTAAAAC | Hybridized to lacZ |